Chapitre D'ouvrage Année : 2021

Fluorescence Spectrometry

Résumé

Fluorescence spectrometry (FLS) is a fundamental toolbox that provides in-depth information about the electronic transitions and interactions in a molecular system. With this technique, one can assay the state of a physical, chemical, or biological system by studying their photophysical properties. The electronic transitions or interactions in a particular molecular system are monitored by analyzing the changes in the fluorescent optical properties and their life-times. In this chapter, we have included the fundamental principles of fluorescence spectrometry along with the experimental and working aspects. The concepts of steady-state and time-resolved fluorescence spectroscopy and the modern development in this regime have been discussed in detail. All the aspects of FLS for characterizing the soft-nano composites have been comprised and explained. The future perspectives and modern applications of FLS in physical, chemical and medical sciences along with the bio-imaging have been covered within the scope of this chapter.

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Dates et versions

hal-04471028 , version 1 (21-02-2024)

Identifiants

Citer

Dharmendra Pratap Singh, Sanjeev Inamdar, Sandeep Kumar. Fluorescence Spectrometry. Dheeraj Kumar Singh; Manik Pradhan; Arnulf Materny. Modern Techniques of Spectroscopy, 13, Springer Singapore, pp.431-468, 2021, Progress in Optical Science and Photonics (POSP, volume13), 978-981-33-6083-9. ⟨10.1007/978-981-33-6084-6_17⟩. ⟨hal-04471028⟩
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