Purification and Characterization of an Endo-N-Acetyl-4-D-Glucosaminidase from the Culture Medium of Stigmatella aurantiaca DW4 - Université du Littoral Côte d'Opale Access content directly
Journal Articles Journal of Bacteriology Year : 1994

Purification and Characterization of an Endo-N-Acetyl-4-D-Glucosaminidase from the Culture Medium of Stigmatella aurantiaca DW4

Yannis Karamanos
Thierry Grard

Abstract

A novel endo-N-acetyl- ,-D-glucosaminidase (ENGase), acting on the diN -acetylchitobiosyl part of N-linked glycans, was characterized in the culture medium of Stigmatella aurantiaca DW4. Purified to homogeneity by ammonium sulfate precipitation, gel filtration, and chromatofocusing, this ENGase presents, upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, a molecular mass near 27 kDa. Optimal pH and pI were 4.0 and 6.8, respectively. The enzyme, named ENGase St, exhibits high activity on oligomannoside-type glycoasparagines and glycoproteins and could also hydrolyze hybrid-and complex-type glycoasparagines but does not acts as a murein hydrolase.

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hal-03697251 , version 1 (16-06-2022)

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Sylvain Bourgerie, Yannis Karamanos, Thierry Grard, Raymond Julien. Purification and Characterization of an Endo-N-Acetyl-4-D-Glucosaminidase from the Culture Medium of Stigmatella aurantiaca DW4. Journal of Bacteriology, 1994, 176 (20), pp.6170-6174. ⟨10.1128/jb.176.20.6170-6174.1994⟩. ⟨hal-03697251⟩
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