Host genetic requirements for DNA release of lactococcal phage TP901 ‐1 - Université du Littoral Côte d'Opale Access content directly
Journal Articles Microbial Biotechnology Year : 2022

Host genetic requirements for DNA release of lactococcal phage TP901 ‐1

Sofía Ruiz-Cruz
Andrea Erazo Garzon
  • Function : Author
Philip Kelleher
  • Function : Author
Francesca Bottacini
  • Function : Author
Solvej Østergaard Breum
  • Function : Author
Horst Neve
  • Function : Author
Knut Heller
  • Function : Author
Finn Vogensen
  • Function : Author
Simon Palussière
  • Function : Author
Pascal Courtin
  • Function : Author
Marie‐pierre Chapot-Chartier
  • Function : Author
Evgeny Vinogradov
  • Function : Author
Jennifer Mahony
Douwe van Sinderen


The first step in phage infection is the recognition of, and adsorption to, a receptor located on the host cell surface. This reversible host adsorption step is commonly followed by an irreversible event, which involves phage DNA delivery or release into the bacterial cytoplasm. The molecular components that trigger this latter event are unknown for most phages of Gram-positive bacteria. In the current study, we present a comparative genome analysis of three mutants of Lactococcus cremoris 3107, which are resistant to the P335 group phage TP901-1 due to mutations that affect TP901-1 DNA release. Through genetic complementation and phage infection assays, a predicted lactococcal three-component glycosylation system (TGS) was shown to be required for TP901-1 infection. Major cell wall saccharidic components were analysed, but no differences were found. However, heterologous gene expression experiments indicate that this TGS is involved in the glucosylation of a cell envelope-associated component that triggers TP901-1 DNA release. To date, a saccharide modification has not been implicated in the DNA delivery process of a Gram-positive infecting phage.

Dates and versions

hal-04049596 , version 1 (28-03-2023)



Sofía Ruiz-Cruz, Andrea Erazo Garzon, Philip Kelleher, Francesca Bottacini, Solvej Østergaard Breum, et al.. Host genetic requirements for DNA release of lactococcal phage TP901 ‐1. Microbial Biotechnology, 2022, 15 (12), pp.2875-2889. ⟨10.1111/1751-7915.14156⟩. ⟨hal-04049596⟩
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